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腎素是參與體內腎素-血管緊張素系統的一種酶,它可以介導胞外體積和動脈血管的收縮。它調節血壓和電解質平衡。血管緊張素II可誘導血管壓縮并導致血壓升高。它也能增加ADH和醛固酮的分泌,刺激下丘腦來激活饑渴反應。
產品描述:
腎素是參與體內腎素-血管緊張素系統的一種酶,它可以介導胞外體積和動脈血管的收縮。它調節血壓和電解質平衡。血管緊張素II可誘導血管壓縮并導致血壓升高。它也能增加ADH和醛固酮的分泌,刺激下丘腦來激活饑渴反應。過度活化腎素-血管緊張素系統導致血管收縮和保持鈉和水。腎素已經被證實是治療高血壓一個顯著指標。
產品優勢:
Amplite腎素檢測試劑盒提供了一個簡便的實驗方案,利用我們專有的 iFluor 488/TQ 520熒光共振能量轉換肽,高通量的篩選腎素抑制劑或檢測腎素的活性。在熒光共振能量轉換肽中,iFluor 488熒光性被TQ 520所淬滅。在裂解成兩個獨立片段后,iFluor 488熒光性被恢復,這種熒光信號很容易被熒光酶標儀(Ex/Em=490/520nm)所測定。這個實驗比基于EDANS/DABCYL實驗大約靈敏50倍。Amplite腎素檢測試劑盒能檢測到1ng/100ul的腎素。
英文注釋:
Our Amplite Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activity. Cleavage of LLVY-R110 by proteasome generates the strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation of 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays for evaluation of proteasome activity or inhibitor screening in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. The proteasomal degradation pathway is essential for many cellular processes, including the cell cycle, the regulation of gene expression, and responses to oxidative stress. The most common form of the proteasome in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps. The 20S core contains three major proteolytic activities including chymotrypsin-like, trypsin-like and caspase-like. It is responsible for the breakdown of key proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control.
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