13456 AAT Bioquest 20S蛋白酶體活性檢測試劑盒
參考價 | 面議 |
- 公司名稱 天津百螢生物科技有限公司
- 品牌
- 型號 13456
- 所在地 天津市
- 廠商性質 生產廠家
- 更新時間 2015/11/19 16:00:00
- 訪問次數 497
參考價 | 面議 |
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蛋白酶體zui通用的形式就是26s蛋白酶體,它包含一個20S的核心結構顆粒和兩個19S調節調節基團。所有20S的核心結構顆粒包含四對七聚物環狀結構組成兩種不同的亞基,alpha亞基是結構亞基,beta亞基主要是催化亞基。
產品描述:
蛋白酶體zui通用的形式就是26s蛋白酶體,它包含一個20S的核心結構顆粒和兩個19S調節調節基團。所有20S的核心結構顆粒包含四對七聚物環狀結構組成兩種不同的亞基,alpha亞基是結構亞基,beta亞基主要是催化亞基。在兩個環狀結構外每個含有七個alpha亞基主要是為了調整顆粒的??俊lpha亞基N端結構是可以阻擋未調節底物進入內腔的門戶。內腔兩個環分別包含七個beta亞基包含酶激活位點來執行蛋白水解反應。Amplite 20S蛋白酶體熒光檢測試劑盒可以用來檢測培養細胞或者細胞裂解液里蛋白酶體復合物中類似*蛋白酶的活性。這個試劑盒使用LLVY-R110作為蛋白酶的熒光指示劑。通過蛋白酶切開LLVY-R110產生強綠色的R110,其檢測熒光在520-530nm,激發光為480-500nm。該試劑盒提供所有必要成分并提供*實驗操作方案。這個實驗能穩定并高通量的評估從培養細胞或者溶液中蛋白酶體的活性和篩選抑制劑。該實驗方案能方便的在96或384微孔板中分析。
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英文注釋:
Our Amplite Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activity. Cleavage of LLVY-R110 by proteasome generates the strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation of 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays for evaluation of proteasome activity or inhibitor screening in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. The proteasomal degradation pathway is essential for many cellular processes, including the cell cycle, the regulation of gene expression, and responses to oxidative stress. The most common form of the proteasome in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps. The 20S core contains three major proteolytic activities including chymotrypsin-like, trypsin-like and caspase-like. It is responsible for the breakdown of key proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control.
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